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SKU: OKVC00001 Categories: ,


Predicted Species ReactivityHuman
Kit PrincipleAviva Systems Biology Covid-19 IgA ELISA Kit (OKVC00001) is a two-step incubation, solid-phase ELISA assay in which microwell strips were coated with SARS-CoV-2 recombinant antigens. The serum/plasma specimen is added, and during the first incubation, all anti SARS-CoV-2 antibodies if present in the specimen will bind to the recombinant antigens precoated on the wells. After washing out all the other substances of the specimen SARS-CoV-2 specific IgA antibodies bound to the solid phase are detected by the addition of anti human IgA conjugated to the enzyme horseradish peroxidase (HRP) to form an antigen- antibody-antibody-HRP enzyme complex on the microwell surface. Upon addition of TMB substrate, the HRP enzyme converts it into a blue color signal. The intensity of the color is directly proportional to the concentration of SARS-Cov-2 IgA antibodies in the specimen.
Reconstitution and StorageStore COVID-19 IgA ELISA Kit as indicated in product manual.
Sample TypeClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Target Reference1. Pr. Yazdan Yazdanpanah, Chair of GloPID R @GloPID_R, 2019 Novel Coronavirus Global Research and Innovation Forum: Towards a Research Roadmap.
2. Wang W, Xu Y, Gao R, et al. Detection of SARS-CoV-2 in Different Types of Clinical Specimensexternal icon. JAMA. Published online March 11, 2020. DOI:10.1001/jama.2020.3786.
3. Guan WJ, Ni ZY, Hu Y, et al. Clinical Characteristics of Coronavirus Disease 2019 in Chinaexternal icon. N Engl J Med. February 28, 2020. DOI: 10.1056/NEJMoa2002032.
4. Kakimoto K, Kamiya H, Yamagishi T, et al. Initial Investigation of Transmission of COVID-19 Among Crew Members During Quarantine of a Cruise Ship – Yokohama, Japan, February 2020. MMWR Morb Mortal Wkly Rep. ePub: March 17, 2020. DOI: iconexternal icon.
Assay InfoQualitative two-step solid-phase ELISA
Intended UseCovid-19 IgA ELISA is an enzyme-linked immunosorbent assay for the qualitative detection of IgA-class antibodies to SARS-CoV-2 virus in human serum or plasma.
WarningThe test kit does not contain any viable infectious agents. However all samples must be considered as potentially infectious.
Do not ingest the reagents. Avoid contact with eyes, skin and mucose. Wear protective clothing and disposable gloves. Do not allow smoking or eating where antigen containing materials are being handled.
Preclude any pipetting by mouth.
Handle all samples and test components as though capable of transmitting infection. Clean spills thoroughly using an appropriate intermediate-to high level disinfectant. Decontaminate and dispose of specimens and all potentially contaminated materials as if they contain infectious agents.
Avoid splashing or aerosol formation.
Avoid contacting Substrate Reagent or Stop solution with the skin or other mucose membranes. In case of contact, wash thoroughly with water.
IntroductionCoronavirus disease 2019 (COVID-19) is a respiratory disease caused by infection with the SARS-CoV-2 virus(1,2). Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In severe cases, infection can cause pneumonia, severe acute respiratory syndrome (SARS), kidney failure and death(3). Coronaviruses (CoV) are a large family of RNA viruses that cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARSCoV). The 2019 Novel Coronavirus, formerly known as 2019-nCoV and now known as SARS-COV-2, is a new strain of coronavirus that was first identified during an outbreak in Wuhan, China which started in December 2019(4).
Gene SymbolSARS-COV-2
Twelve (12) 1×8-well strips coated with SARS-CoV-2 recombinant proteins. The strip are packaged in a strip holder and sealed in the foiled envelope with desiccant.12 1×8-well strips
Sample diluent11 ml
Negative control0.5 ml
Positive control (Deactivated, not infective)0.5 ml
Anti human IgA- HRP conjugates6 ml
Wash Buffer (30x concentrate)25 ml
TMB substrate11 ml
Stop solution11 ml
Product insert1 set


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