The Glucagon ELISA kit measures Glucagon (1-29) with no detectable cross-reactivity to Glicentin, Oxyntomodulin, GLP-1, GLP-2, or GRPP. Simple procedure, 2.5 hour incubations, no extraction needed, no special collection tube required. Relevant dynamic range and sensitivity.
Glucagon and Oxyntomodulin, a peptide hormone secreted by the alpha cells of pancreas, share identical amino acid sequence in the N-terminal 29 aa. Glucagon is a 29-amino acid polypeptide processed from proglucagon in pancreatic alpha cells.1 In intestinal L-cells proglucagon is cleaved into glicentin, corresponding to proglucagon residues no 1-69. Glicentin can further be processed into oxyntomodulin, corresponding to proglucagon residues no 33-69. These peptides are released simultaneously upon stimulation.
Glucagon has shown to have an effect opposite to that of insulin, i.e. it raises blood glucose levels. It causes the liver to convert glycogen into glucose, which is then released into the blood stream.2-4 During hypoglycaemia, glucagon secretion offers a protective feedback mechanism, defending the organism against damaging effects of glucose deficiency in the brain and nerves.5
|Regulatory Status||For in vitro diagnostic use.|
|Packaging||96 well microtiter|
|Detection||HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter|
|Dynamic Range||6, 7-314 pg/mL|
|Limit of Detection||2.4 pg/mL|
|Sample Size||50 µL|
|Assay Time||2.5 hours|
|Species Reactivity||Human, Mouse|
|Shelf Life||24 months|